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rhil 15  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec rhil 15
    Rhil 15, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rhil+15/Human+IL-15%2C+premium+grade/pmc13292952-174-57-62
    Average 96 stars, based on 176 article reviews
    rhil 15 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Breast cancer-on-chip for patient-specific efficacy and safety testing of CAR-T cells.
    Article Snippet: .. T cells were cultured for 3 days in X-VIVO 15 medium (BE02-060F; Lonza) supplemented with 5 ng/mL of rhIL-15 (130-093-955; Miltenyi Biotec), 1X Glutamax and 100 U/mL penicillin-streptomycin. ..

    Article Title: Harnessing skin-resident γδ T cells for immunotherapy in cutaneous squamous cell carcinoma
    Article Snippet: Human skin–derived T lymphocytes, isolated from healthy human donor skins, were provided by GammaDelta Therapeutic and cultured in γδ T cells expansion media composed of cTexMacs medium, containing TexMacs medium (Miltenyi Biotec, catalog no. 130-097-196), 10% plasma derived pooled human serum (One Lambda, catalog no. A25761 ) and 1% penicillin/streptomycin (Sigma-Aldrich, catalog no. P0781), supplemented with a proprietary recombinant human cytokine cocktail to support Vδ1 + γδ T cell survival and proliferation. .. For phenotypic characterization, human skin–derived T lymphocytes, containing a fraction of Vδ1 + γδ T cells (stated in the respective legends), were cultured in presence of limited cytokine support and injected into SCC-bearing mice intravenously when tumors reached a volume between 100 and 200 mm 3 . rhIL-2 (0.5 μg per mouse, BioLegend, catalog no. 589108) and rhIL-15 (1 μg per mouse, Miltenyi Biotec, catalog no. 130-095-766) were i.p. injected daily for 7 or 14 days. ..

    Article Title: IKAROS and AIOLOS directly regulate AP-1 transcriptional complexes and are essential for NK cell development.
    Article Snippet: .. Labeled cells were cultured in either 5 or 25 ng ml−1 rhIL-15 (Miltenyi Biotec) for up to 144 h. In STAT5 signaling studies, expanded NK cells were first starved of IL-15 for at least 4 h and then reexposed to 50 ng ml−1 rhIL-15. pSTAT5 levels were measured as described in 30-min intervals over 2 h. ..

    Recombinant:

    Article Title: Baboon endogenous retrovirus (ERV) envelope pseudotyped lentiviral vectors outperform human ERV lentivectors for transduction of T, B, NK and HSPCs
    Article Snippet: .. Peripheral blood (PB) T lymphocytes were prestimulated for 3 days with T cell human hCD3/hCD28 TransAct beads (Miltenyi Biotec; #130-111-160,) supplemented with IL-2 (100 ng/ml) (Miltenyi Biotec; #130-097-743,) in RPMI or were prestimulated with recombinant 10 ng/mL human rIL-7 (rhIL-7; Miltenyi Biotec, #130-093-937) and 10 ng/ml rhIL-15 (Miltenyi Biotec 130-093-955,) for 3 days. .. Next, 5E4 PB T cells upon TCR or cytokine stimulation were seeded in 48-well plates coated with RetroNectin® (Takara; #T100B; 12 μg/ml PBS according to manufacturer’s recommendations) and transduced with LVs at the indicated multiplicity of infection (MOI).

    Article Title: Baboon endogenous retrovirus (ERV) envelope pseudotyped lentiviral vectors outperform human ERV lentivectors for transduction of T, B, NK and HSPCs.
    Article Snippet: .. Peripheral blood (PB) T lymphocytes were prestimulated for 3 days with T cell human hCD3/hCD28 TransAct beads (Miltenyi Biotec; #130-111- 160,) supplemented with IL-2 (100 ng/ml) (Miltenyi Biotec; #130-097- 743,) in RPMI or were prestimulated with recombinant 10 ng/mL human rIL-7 (rhIL-7; Miltenyi Biotec, #130-093-937) and 10 ng/ml rhIL-15 (Miltenyi Biotec 130-093-955,) for 3 days. .. Next, 5E4 PB T cells upon TCR or cytokine stimulation were seeded in 48- well plates coated with RetroNectin® (Takara; #T100B; 12 μg/ml PBS according to manufacturer’s recommendations) and transduced with LVs at the indicated multiplicity of infection (MOI).

    Modification:

    Article Title: IKAROS and AIOLOS directly regulate AP-1 transcriptional complexes and are essential for NK cell development.
    Article Snippet: NK cells were purified using the EasySep Mouse NK isolation Kit (Stem Cell Technologies, 19855), per the manufacturer’s guidelines. .. Cells were maintained at 37 °C and 5% CO2 in Iscove’s Modified Dulbecco’s Medium (IMDM; Gibco; 12440061) supplemented with 10% (vol/vol) fetal calf serum (FCS; In Vitro Technologies; MORFBSAU), 2 mM GlutaMax (Gibco; 35050061), 50 μM β-mercaptoethanol, 100 μg ml−1 streptomycin, 100 IU ml−1 penicillin (Gibco; 15070063) and 50 ng ml−1 rhIL-15 (Miltenyi Biotec; 130-095-765). .. The murine B16F10 melanoma cell line (American Type Culture Collection) was cultured in DMEM containing 10% (vol/vol) FBS, 50 mM β-mercaptoethanol (Sigma-Aldrich), 100 mg ml−1 streptomycin and 100 IU ml−1 penicillin (Sigma-Aldrich).

    In Vitro:

    Article Title: IKAROS and AIOLOS directly regulate AP-1 transcriptional complexes and are essential for NK cell development.
    Article Snippet: NK cells were purified using the EasySep Mouse NK isolation Kit (Stem Cell Technologies, 19855), per the manufacturer’s guidelines. .. Cells were maintained at 37 °C and 5% CO2 in Iscove’s Modified Dulbecco’s Medium (IMDM; Gibco; 12440061) supplemented with 10% (vol/vol) fetal calf serum (FCS; In Vitro Technologies; MORFBSAU), 2 mM GlutaMax (Gibco; 35050061), 50 μM β-mercaptoethanol, 100 μg ml−1 streptomycin, 100 IU ml−1 penicillin (Gibco; 15070063) and 50 ng ml−1 rhIL-15 (Miltenyi Biotec; 130-095-765). .. The murine B16F10 melanoma cell line (American Type Culture Collection) was cultured in DMEM containing 10% (vol/vol) FBS, 50 mM β-mercaptoethanol (Sigma-Aldrich), 100 mg ml−1 streptomycin and 100 IU ml−1 penicillin (Sigma-Aldrich).

    In Vivo:

    Article Title: Targeting PD-1 + T cells with chimeric antigen receptors to reduce the HIV reservoir
    Article Snippet: Medium was supplemented with recombinant human IL-2 (30 IU/ml; rhIL-2, Miltenyi Biotec, Bergisch-Gladbach, Germany, catalog no. 130-097-746) for T cells or CD4 + T cells, 100 IU/ml for CD8 + T cells when expanded alone. .. For the in vivo experiments, the T cell medium was enriched with rhIL-7 (5 ng/ml; Miltenyi Biotec, Bergisch-Gladbach, Germany, catalog no. 130-095-362), rhIL-15 (5 ng/ml; Miltenyi Biotec, Bergisch-Gladbach, Germany, catalog no. 130-095-764), and IL-2 (30 IU/ml). ..

    Injection:

    Article Title: Harnessing skin-resident γδ T cells for immunotherapy in cutaneous squamous cell carcinoma
    Article Snippet: Human skin–derived T lymphocytes, isolated from healthy human donor skins, were provided by GammaDelta Therapeutic and cultured in γδ T cells expansion media composed of cTexMacs medium, containing TexMacs medium (Miltenyi Biotec, catalog no. 130-097-196), 10% plasma derived pooled human serum (One Lambda, catalog no. A25761 ) and 1% penicillin/streptomycin (Sigma-Aldrich, catalog no. P0781), supplemented with a proprietary recombinant human cytokine cocktail to support Vδ1 + γδ T cell survival and proliferation. .. For phenotypic characterization, human skin–derived T lymphocytes, containing a fraction of Vδ1 + γδ T cells (stated in the respective legends), were cultured in presence of limited cytokine support and injected into SCC-bearing mice intravenously when tumors reached a volume between 100 and 200 mm 3 . rhIL-2 (0.5 μg per mouse, BioLegend, catalog no. 589108) and rhIL-15 (1 μg per mouse, Miltenyi Biotec, catalog no. 130-095-766) were i.p. injected daily for 7 or 14 days. ..

    Labeling:

    Article Title: IKAROS and AIOLOS directly regulate AP-1 transcriptional complexes and are essential for NK cell development.
    Article Snippet: .. Labeled cells were cultured in either 5 or 25 ng ml−1 rhIL-15 (Miltenyi Biotec) for up to 144 h. In STAT5 signaling studies, expanded NK cells were first starved of IL-15 for at least 4 h and then reexposed to 50 ng ml−1 rhIL-15. pSTAT5 levels were measured as described in 30-min intervals over 2 h. ..



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